Genetic Analysis Facility Department of Biology, NMSU

The ABI Prism 3100 ABI Genetic Analyzer is an automated capillary electorphoresis system that can separate, detect, and analyze up to 16 capillaries of fluorescently labeled DNA fragments in one run. It offers continuous operation with automated polymer and sample injection, separation and detection, and data analysis. The 3100 is a machine capable of running sequence and fragment analysis.

 

What the Instrument Does

DNA Analysis	Purpose
Sequencing analysis	Separates a mixtures of DNA fragments according to length Provides a profile of the separation Determines the order of the four bases
Fragment analysis	Separates a mixtures of DNA fragments according to length Provides a profile of the separation Determines the length of each fragment Estimates the relative concentration of each fragment in the sample

 

Acknowledgements

Purchase of the ABI Prism® 3100 Genetic Analyzer was supported by and award from NSF Division of Biological Instrumentation to Dr. Michele Nishiguchi (PI), Dr. Brook Milligan, Dr. Daniel Howard, Dr. Peter Houde, Dr. Geoffrey Smith, and Dr. Kevin Oshima of the Department of Biology (DIB #0079820).