BIOL 211-CELLULAR AND ORGANISMAL BIOLOGY
Topic: DNA replication
Chapter 12 section 12.3
Figures used in lecture: 12.10, 12.12
DNA Replication (again)
Models of DNA Replication
three models were proposed:
Experiment of Meselson and Stahl, 1957
result: ½ DNA is 14N-15N hybrid, ½ DNA is 14N-14N light density
This experiment indicates that the semiconservative model of DNA replication is correct.
Define/explain: origin of replication (ORI), parental strands of DNA, daughter strands of DNA, Okazaki fragments
DNA replication involves many enzymes including helicase, primase, DNA polymerase, DNA ligase
You should be able to
1. Explain each of the proposed models for DNA replication being able to propose the results of the Meselson & Stahl experiment for DNA that is replicating either by a conservative method or a dispersive method.
2. List and explain the steps of DNA replication
3. List the enzymes and their function