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New Mexico State University

Kathryn Hanley
kathryn hanley

Kathryn A. Hanley


Title: Assistant Professor
Research area: Virus Evolutionary Ecology
Office location: FH 479
Laboratory Location: FH 455
Email Address: khanley@nmsu.edu
Office Phone: 575-646-4583
Lab Phone: 575-646-4791
Lab Webpage: http://biology-web.nmsu.edu/hanley/


Education:

  • Postdoc - Laboratory of Infectious Diseases, National Institutes of Health 1999-2004
  • Postdoc- Biology Department, University of Maryland 1997-99
  • Postdoc- Section of Evolution and Ecology, University of California, Davis 1994-96
  • Ph.D.- Biology, University of California, San Diego, 1994
  • B.A. - Biology, Amherst College, 1989

Courses Taught:

  • Evolutionary Ecology (BIOL 569)
  • Biology of Emerging Infectious Diseases (BIOL 450/550)
  • Virology (BIOL 475)
  • Human Biology (BIOL 101)
  • Epidemiology for Molecular Biologists (MOLB 450)

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Research Interests:
Research in the Hanley lab centers on the biology of arthropod-borne viruses (arboviruses), particularly mosquito-transmitted dengue virus (genus Flavivirus, family Flaviviridae), the agent of dengue fever.  Our goal is to understand how interactions between arboviruses and their vectors may affect virus emergence, geographic spread, disease potential, and control.  At present research in the lab focuses on four major questions: (i) What are the mechanisms the regulate emergence of sylvatic strains of dengue, which are maintained in non-human primates and arboreal Aedes mosquitoes in the forests of Asia and West Africa, into circulation between humans and peridomestic Aedes?, (ii) How do antiviral immune responses in mosquitoes, specifically RNA interference, affect virus evolution, and more specifically what counter-measures do arboviruses employ to suppress RNA interference?, (iii) What is the role of the untranslated regions in flaviviruses in viral replication in both vector and human, and how can mutations in this region be used to generate live attenuated dengue virus vaccines?, (iv) What is the strength and symmetry of competition between different strains, ecotypes and serotypes of dengue virus, and how does such competition influence the epidemiology of dengue disease?  Students in the lab employ a wide variety of techniques drawn from molecular virology, entomology, evolutionary biology and ecology to address these questions.

Selected Publications:

  • Pepin, K.M. and Hanley K.A. 2008. Density-dependent competitive suppression of sylvatic dengue virus by endemic dengue virus in cultured mosquito cells. submitted to Vector Borne and Zoonotic Diseases.
  • Vasilakis, N., E.B. Fokam, C.T.Hanson, E. Weinberg, A.A. Sall, S. S. Whitehead, K.A. Hanley, S.C. Weaver. 2008. Genetic and phenotypic characterization of sylvatic dengue strains. submitted to Virology
  • Romero, T.A., J.E. Blaney, Jr., S.S. Whitehead and K.A. Hanley. 2008. Design of dengue virus vaccines: analysis of the impact of deletion mutations in the 3’ untranslated region on RNA secondary structure. submitted to Virology.
  • Hanley, K.A., J.T. Nelson, E. E. Schirtzinger, S.S. Whitehead, C.T. Hanson. 2008. Superior infectivity for mosquito vectors contributes to competitive displacement among strains of dengue virus. BMC Ecology 8:1.[PDF]
  • Pepin, K.M., K. Lambeth, K.A. Hanley. 2008. Asymmetric competitive suppression between strains of dengue virus. BMC Microbiology 8:28.[PDF]
  • Blaney, J.E., Jr., N. Sathe, C.T. Hanson. L. Goddard, T.A. Romero, K.A. Hanley, B.R. Murphy, S.S. Whitehead. 2008. Dengue virus type 3 vaccine candidates generated by introduction of deletions in the 3’ untranslated region (UTR) or exchange of the DENV3 3’ UTR with that of DENV4. Vaccine 26:817-28.
  • Vasilakos, N., E. J. Shell, E.B. Fokam, P.W. Mason, K.A. Hanley, D.M. Estes, and S.C. Weaver. 2006. Potential of ancestral sylvatic dengue-2 viruses to re-emerge. Virology 358, 402-412.[PDF]
  • T. A. Romero, E. Tumban, J. Jun, W.B.Lott, and K.A. Hanley. 2006. Secondary structure of dengue virus 4 3' untranslated region: Impact of deletion and substitution mutations. Journal of General Virology 87, 3291-3296. [PDF]
  • Hanley, K.A., L.B. Goddard, L.E. Gilmore, T.W. Scott, J. Speicher, B.R. Murphy, A. G. Pletnev. 2005. Infectivity of West Nile/Dengue chimeric viruses for West Nile and Dengue mosquito vectors. Vector-Borne and Zoonotic Diseases 5: 1-10.[PDF]
  • Blaney, Jr., J.E., C.T. Hanson, C. Y. Firestone, K.A. Hanley, B.R. Murphy, S.S. Whitehead. 2004. Genetically modified, live attenuated dengue virus type 3 vaccine candidates. The American Journal of Tropical Medicine and Hygiene 71: 811-821. [PDF]
  • Blaney, J.E., Jr., C.T. Hanson, K.A. Hanley, B.R. Murphy and S. W. Whitehead. 2004. Vaccine candidates derived from a novel infectious cDNA clone of an American genotype dengue virus type 2. Biomed Central Infectious Diseases 4: 39-49. [PDF]
  • Hanley, K.A., L.R. Manlucu, G.G. Manipon, C.T. Hanson, S.S. Whitehead, B.R. Murphy, J.E. Blaney Jr. 2004. Introduction of mutations into the non-structural genes or 3’ untranslated region of an attenuated dengue virus type 4 vaccine candidate further decreases replication in rhesus monkeys while retaining protective immunity. Vaccine 22:3440-3448.
  • Burch, C.L., P.E. Turner, and K.A. Hanley. 2003. Patterns of epistasis in RNA viruses: a review of the evidence from vaccine design. Journal of Evolutionary Biology 16:1223-1235.
  • Whitehead, S.S., K.A. Hanley, J.E. Blaney Jr., L.E. Gilmore, W.R. Elkins, and B.R. Murphy. 2003. Substitution of the structural genes of dengue virus type 4 with those of type 2 results in chimeric vaccine candidates which are attenuated for mosquitoes, mice and rhesus monkeys. Vaccine 21:4307-4316.
  • Hanley, K.A., L.R. Manlucu, L.E. Gilmore, J.E. Blaney Jr., C. T. Hanson, B.R. Murphy and S.S. Whitehead. 2003. A trade-off in replication in mosquito versus mammalian systems conferred by a point mutation in the NS4B protein of dengue virus type 4. Virology 312: 222-232.
  • Whitehead, S.S., B. Falgout, K.A. Hanley, J.E. Blaney Jr., L. Markoff, and B.R. Murphy. 2003. A live attenuated dengue virus type 1 vaccine candidate with a 30 nucleotide deletion in the 3’ untranslated region is highly attenuated and immunogenic in monkeys. Journal of Virology 77:1653-1657.
  • Hanley, K.A., J.J. Lee, J.E. Blaney, Jr., B.R. Murphy, and S.S. Whitehead. 2002. Paired charge-to-alanine mutagenesis of dengue virus type 4 NS5 confers temperature-sensitive, host-range and mouse attenuation phenotypes. Journal of Virology 76: 525-531.
  • Hanley, K.A. and J.A. Stamps. 2002. Does corticosterone mediate bidirectional interactions between social behaviour and blood parasites in juvenile black iguanas, Ctenosaura similis? Animal Behaviour 63: 311-322.
  • Troyer, J. M.1, K.A. Hanley1, S.S. Whitehead, D. Strickman, R.A. Karron, A. P. Durbin, and B. R. Murphy. 2001. A live attenuated recombinant dengue-4 virus vaccine candidate with restricted capacity for dissemination in mosquitoes and lack of transmission from vaccinees to mosquitoes. The American Journal of Tropical Medicine and Hygiene 65:414-419. 1Equal contribution by first two authors.
  • Pletnev, A.G., M. Bray, K.A. Hanley, J. Speicher and R. Elkins. 2001. Tick-borne Langat/mosquito-borne Dengue flavivirus chimera, a candidate live-attenuated vaccine for protection against disease caused by members of the tick-borne encephalitis virus complex: evaluation in rhesus monkeys and mosquitoes. Journal of Virology 75: 8259-8267. [PDF]
  • Chao, L., K.A. Hanley, C. L. Burch, C. Dahlberg, and P.E. Turner. 2000. Kin selection and the evolution of virulence in parasites: Making hard and soft choices. Quarterly Review of Biology 75:261-275. [PDF]
  • Schall, J.J., H. R. Prendiville, and K.A. Hanley. 2000. Prevalence of the tick, Ixodes pacificus, on western fence lizards, Sceloporus occidentalis: Trends by gender, size, season, site and mite infestation. Journal of Herpetology 34:160-163.
  • K.A. Hanley, M.L. Elliott, and J.A. Stamps. 1999. Chemical recognition of familiar versus unfamiliar conspecifics by juvenile black iguanas, Ctenosaura similis. Ethology 105: 641-650.
  • Turner, P.E., C. L. Burch, K.A. Hanley, and L. Chao. 1999. Hybrid frequencies confirm limit to coinfection in the RNA bacteriophage Phi 6. Journal of Virology 73: 2420-2424. [PDF]
  • Hanley, K.A., K.R. Petren, and T.J. Case. 1998. An experimental investigation of the competitive displacement of a native gecko by an invading gecko: no role for parasites. Oecologia 115: 196-205.
  • Hanley, K.A., R.N. Fisher, and T.J. Case. 1995. Higher mite infestations in sexual geckos than their asexual congeners. Evolution 49(3): 418-436.
  • Hanley, K.A., D.M. Vollmer, and T.J. Case. 1995. The distribution and prevalence of helminths, coccidia and blood parasites in two competing species of gecko: implications for apparent competition. Oecologia 102: 220-229.
  • Radtkey, R.R., S. Donnellan, R.N. Fisher, C. Moritz, K.A. Hanley, and T.J. Case. 1995. When species collide: the origin and spread of an asexual gecko species. Proceedings of the Royal Society of London (Biology) 259:145-152.
  • Upton, S.J., K.A. Hanley, and T.J. Case. 1994. Eimeria frenatus sp. n. and Eimeriarochalimai (Apicomplexa: Eimeriidae) from Hemidactylus frenatus (Sauria:Gekkonidae) in Hawaii. Transactions of the American Microscopical Society113(3): 390-394.
  • Hanley, K.A., D.T. Bolger, and T.J. Case. 1994. Comparative ecology of sexual and asexual gecko congeners (Lepidodactylus) in French Polynesia. Evolutionary Ecology 8: 438-454.
  • Upton, S.J., K.A. Hanley, T.J. Case, and C.T. McCallister. 1991. Description of Isospora schlegeli (Apicomplexa: Eimeriidae) from gekkonid lizards in the South Pacific. Canadian Journal of Zoology 69: 3108-3110